Table 1 Primer pairs derived from the chloroplast genome of G. tenuistipitata var. liui
Name | Forward primer (5′-3′) | Reverse primer (5′-3′) | Repeat motif | Predicted product size (bp) | Genomic coordinates of the SSR loci | Ta(°C) | Coding/non-coding region |
|---|---|---|---|---|---|---|---|
GT1 | TTTATCAACGATCCCTGTAG | AATGGACTGTAATTCACCAA | (T)10 | 336 | 4314 – 4323 | 52 | Non-coding |
GT2 | TTTTTGAGCGATATTTTGAC | AGAATAAGACCACCTGAACC | (T)10 | 310 | 78590 – 78599 | 53 | Coding |
GT3 | CCATAATGGAGATCTGTTTG | CTGGCAACATAGTTAGCATT | (T)10 | 306 | 84904 – 84913 | 53 | Coding |
GT4 | AGCAATCCTAAATTGACAAC | AAGGTAGACCAGGAGAAAAA | (ATT)5 | 295 | 99538 – 99552 | 53 | Coding |
GT5 | AGAAATTGATCAAGCTGTT | TTTTCAGCAATTGGAGTATC | (AT)8 | 329 | 105129 – 105144 | 52 | Non-coding |
GT6 | CCTACAATCAGAATGGAATG | AGCTTCCAAGAAAAATGAGT | (A)10 | 303 | 143312 – 143321 | 53 | Coding |
GT7 | ATCCTTCTTTTAAGCCGTAG | TCTTCCATGAAGTCTTCTTTT | (T)10 | 309 | 161988 – 161997 | 53 | Non-coding |
GT8 | CTCCTGACATGATAAACACC | CTTCGATTTGTTTAATGAGC | (AT)7 | 288 | 179473 – 179486 | 52 | Non-coding |