SDS-PAGE and western blot analysis of protein fractions from the IMAC purification of his
-thioredoxin-GSL1. A: SDS-PAGE analysis of the fractions obtained. Lane 1: flow through from column; lane 2: eluate after first wash; lanes 3-9, fractions after elution buffer was applied. The arrow indicates the GSL1 fusion protein. B: Western blot analysis of the same protein fractions using an anti-thioredoxin antibody.