Figure 4

Western blotting results of PLCγ2 splenic B cell. The representative results of western blotting were displayed. Splenic B cells were stimulated with anti-IgM. All experiments were replicated and confirmed three times at least. “n” indicates the number of total tested sample for each protein. (A) The phosphorylation of Syk (Tyr525/526; pSyk), Lyn (Tyr507; pLyn), Btk (Tyr223; pBtk), and CD19 (Tyr531; pCD19) sites and (B) Tyr1217 and Tyr759 phosphorylation of PLCγ2 were demonstrated with “Total” as the controls, which indicate the amount of each applied protein. (C) The expressions of NFAT1 and NFAT2 were indicated with β-actin. (D) The phosphorylation of SAPK/JNK (Thr183/Tyr185; pJNK) and ATF-2 (Thr71; pATF-2) were indicated with β-actin. Note that there were two bands for JNK in 54 and 46 kDa due to isoforms as noted by arrows. The ratio of expression level of each sample was calculated by using ImageJ.