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Figure 1 | BMC Research Notes

Figure 1

From: Haplotype defined by the MLH1-93G/A polymorphism is associated with MLH1 promoter hypermethylation in sporadic colorectal cancers

Figure 1

BiPS and MSP analysis of MLH1 promoter region. Upper panel: BiPS analysis of MLH1 promoter region A is shown. U indicates control unmethylated normal DNA; M indicates control methylated DNA. Cancer tissue (T) from cases JM29 and JM161 displayed a methylated band in region A. After SSCP analysis, the extra bands were cut from the electrophoresed gels, reamplified by PCR and sequenced by the dideoxy sequencing procedure. Results of the direct sequencing are shown in the right panel. Sequences (JM161) with methylated cytosines of CpG dinucleotides in region A are shown. Lower panel: MSP analysis of MLH1 promoter region D and direct sequencing of the amplified DNA fragments. U, control unmethylated normal DNA; M, control methylated DNA. Tumor tissue (T) from case JM161 (full methylation case) also showed a methylated band in promoter region D. In contrast, tumor tissue (T) from case JM29 (partial methylation case) did not show a methylated band in the same region.

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BMC Research Notes

ISSN: 1756-0500

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