Figure 1

BDNF expression is induced by subtoxic levels of oxidative stress in PC12 cells. (A) Differentiated PC12 cells were stimulated with the indicated concentrations of H₂O₂ for 15 h. Cell toxicity was measured by LDH assay. Data shown represent mean ± SEM, tested using a one-way ANOVA with Tukey’s post-test (**p <0.01, n =9). (B) Differentiated PC12 cells were stimulated with the indicated concentration of H₂O₂ for 30 min. Total and phosphorylated proteins were evaluated by western blotting analysis. Three independent experiments were performed and representative data are shown.