BDNF signaling in PC12 cells. (A) Total RNA was extracted from differentiated PC12 cells. The gene expression of each neurotrophin receptors was evaluated by RT-PCR analysis. (B) Differentiated PC12 cells were treated with the indicated amounts of BDNF for 30 min. Total and phosphorylated proteins were evaluated by western blotting analysis. Three independent experiments were performed and representative data are shown. (C, D, E) Densitometric analysis of (B). Data shown represent mean ± SEM, tested using one-way ANOVA with Tukey’s post-test (*p <0.05, **p <0.01, n = 3).