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Table 1 Major mutant phenotypes recovered by visual screening of the TILLING population at the seedling stage

From: Development and evaluation of a cucumber TILLING population

Phenotype

No. of families displaying phenotype

Description

Representative family

Seedling lethality

19

Seedling dies, or fails to develop a root after germination

144, 157

Dwarf

10

Cotyledons smaller, short hypocotyl

422

Necrotic lesions

15

Spontaneous necrotic spots appear on cotyledons

38

Albino

3

White cotyledons

164

Yellow leaf

7

True leaves pale-green or yellow, or mosaic green and yellow

218

Tall seedlings

5

Hypocotyl >2 cm taller than wild type

180

Glabrous

1

Cotyledons lack trichomes

16

Non-serrated leaf

3

Leaf edge appears smooth

189

Dark, narrow cotyledon

1

Dark green, narrow cotyledons

411

Fused cotyledons

1

Cotyledons fused together

714

Small leaf

7

True leaf very small

17

Distorted leaf/cotyledon

5

Irregular organ shape

160, 213

Total mutants/ families screened

77/768

  
  1. Six seedlings per M2 family were sown in trays and inspected at the cotyledon-first true leaf stage. The number of families and examples of specific families that segregate for a given phenotype-class are indicated. About 10% of the families exhibited morphological alterations at the seedling stage.