Table 2 Primer pairs used to generate amplicons for the TILLING screen of six genes, as detailed in the methods
From: Development and evaluation of a cucumber TILLING population
Gene | Amplicon | External primers | Internal primers (M13 tag bold) |
|---|---|---|---|
PDS-3 | pdsB | Pds3for1, CACAGATGACATTCTTCCCAAT | pds3for3, CACGACGTTGTAAAACGAC TCTAACATACCCATAGG |
Pds3rev2, CCTAGTTCTACCCTTTGTTCTTGG | pds3rev4, ATAACAATTTCACACAGG GTTTCATGCTGGCTGCC | ||
pdsD | Pds3for5, GGAAATTTGTCTCAACATGTGTGC | Pds3for7, CACGACGTTGTAAAACGAC TCTCCAAACTAGTGAC | |
Pds3rev6, CTTGTGCCACATGGCTAGAATAG | Pds3rev8, GATAACAATTTCACACAGG CTGCCGGTGATGCTGG | ||
pdsF | Pds3for9, AAGGGGCTCGACTGTTCAGAAA | Pds3for11, CACGACGTTGTAAAACGAC CACTAGAAGACTCAGC | |
Pds3rev10, CTGGTAGTGATTCTCGGTTTCA | Pds3rev12, GATAACAATTTCACACAGG CATCCACCGAAGTAGA | ||
Female | acsA | AcsFor1, GAACTATCTACCATATTCCAACC | AcsFor2, CACGACGTTGTAAAACGAC GTACCTATATACCTCACCTCAACAT |
AcsRev3, CACCAACTCGAAAACCTGGGAGCC | AcsRev1, GGATAACAATTTCACACAGG CCTCGTCTTCGTTACTCCTCTCCT | ||
acsB | AcsFor5, TTGATAGAGATTTGAAATGGAGA | AcsFfor6, CACGACGTTGTAAAACGAC CCGGAGTTGAGATTGTGCCAATTC | |
AcsRev7F, CCGAGTGCACTTTTCTTTTTC | AcsRev5, GGATAACAATTTCACACAGG ATTCCCCCAAATATGGATGATG | ||
RMS-3 | rms3A | Rms3for1, GTGTCACCGTGCATGCAATTGCCG | Rms3for2, GTACACTTCAAATCATAAACGGCTG |
Rms3Rev2, TTCATCTCTCAGTTTTCCTACCTAAT | Rms3Rev1, GGGTTACAAACGCTGGCCTTC | ||
rms3B | Rms3for6, ATTGCAAACATAGCCATCAAAATC | Rms3for7, GTCAAAATTATCATTTCTACGCAGG | |
Rms3rev6, CAAGTAAAAACACAGCTCTCAACCT | Rms3Rev5, GAGTGGATGCTATTCCTTTTCGATG | ||
RMS-4 | rms4A | Rms4for5, CTCTCTCCGTTGCTAAGACAAACCC | Rms4for1, TCCGATTACTGTATCTTCCTGCTCG |
Rms4rev6, CAAACTCACCATTGTTCTCAAACCC | Rms4rev1, CGACAGCGATTCAAGCCCTTGACAA | ||
rms4B | Rms4For4, TTCTCGTGGCCAATCCTCTGAC | Rms4for2, CCATTACCGAGGCTTGCCCTAACCT | |
Rms4rev5, GCCCCACAAATCATTACCACTGCAT | Rms4rev2, GCCCCACAAATCATTACCACTGCAT | ||
rmsC | Rms4G3for7, GGATGGAAACTATGGTGGATATA | Rms4for3, CCAGGTACTCCACCGACGCTGATTG | |
Rms4G3rev7, CCCATGAAAGATTGTGAAATCACA | Rms4rev3, GTGATACAGCTAATCTCAAAGTAAC | ||
Cum-1 | Cum1B | Cum1for1, GCTCTTTTCCTCATCAGGTTAGTG | Cum1for3, ACGACGTTGTAAAACGAC TCTCCTGCTCATTCCAC |
Cum1rev2, GTATACACCAAACTGAGAACCAG | Cum1rev4, GATAACAATTTCACACAGG TACAACCCAGATTCCC | ||
Cum1D | Cum1for5, GATATCAATTAAACCATGCGGGC | Cum1for7, CACGACGTTGTAAAACGAC TGGTATGAAATGGGGG | |
Cum1rev6, GATTATCGGTTTCATCTCCATGG | Cum1rev8, GATAACAATTTCACACAGG CCCAATTCAGACCTTC | ||
SP | spB | SPfor1, GGACAGCACAAGAAAAGGTCAC | SPfor2, CACGACGTTGTAAAACGAC TGCCTCTCTCTCTGCT |
SPrev1, CACATCATTTCTTGCCAATTGTC | SPrev2, GATAACAATTTCACACAGG GGGCATCTTTTGCAAC | ||
spC | SPfor1, GGACAGCACAAGAAAAGGTCAC | SPfor3, CACGACGTTGTAAAACGAC CAACCAATTCCCAAAC | |
SPrev1, CACATCATTTCTTGCCAATTGTC | SPrev3, GATAACAATTTCACACAGG CACTCTCTCTCACCAG |