Figure 3
From: Proteomic analysis of the extraembryonic tissues from cloned porcine fetus at day 35 of pregnancy
Immunohistochemical analysis of antioxidant proteins in the control and SCNT extraembryonic tissue. (A) Catalase expression in the control extraembryonic tissue was mainly localized on the luminal epithelium (LE) and endometrial glandular (EG) cells. Magnification: control and SCNT extraembryonic tissue (x200). Scale bars represent 100 μm. (B) Immunohistochemical analysis of GPX in the control and SCNT extraembryonic tissue. GPX was strongly expressed in the luminal epithelium (LE) of the control extraembryonic tissue. The insect in (a, x200) and (c, x200) showed (b, x400) and (d, x400), respectively. Scale bars represent 100 μm. (C) Western blot analysis of antioxidant enzyme proteins in the control and SCNT extraembryonic tissue. The expression of GPX and Catalase was down-regulated in the SCNT extraembryonic tissue.