Comparison of Pindel results when minimum support for event (M) value is adjusted in one sample TES analysis (A) and three-sample TES analysis (B). Adjusting the M parameter in Pindel improved validation rates. When one TES sample was run, adjusting M to 10 increased the validation rate to 75%. Increasing M to 30 did not have an effect on the validation rate. When three samples were run simultaneously, adjusting M to 30 produced results with the highest validation rate. Adjustments to M must take into account the number of samples as well as the depth of coverage of the target.