GATA4 or GATA6 were eliminated from the intestinal epithelium using Villin-Cre. (A) IHC for either GATA4 or GATA6 (brown nuclear stain) demonstrated that GATA4 and GATA6 protein were eliminated from the intestinal epithelium of mutants at E18.5. Inset shows higher magnification image of starred region. Diagram shows the regions from which tissue was harvested for IHC (red boxes). S, stomach; SI, small intestine; Ce, cecum; LI, large intestine. (B) IHC for GATA6 in GATA4 mutant jejunum confirmed that GATA6 protein was maintained in the absence of GATA4. IHC for GATA4 in GATA6 mutant jejunum confirmed that GATA4 protein was maintained in the absence of GATA6. IHC for GATA4 in GATA6 mutant ileum demonstrated that GATA4 was not ectopically induced in the absence of GATA6. Scale bars, 50 μm. All experiments performed using E18.5 embryos.