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Figure 7 | BMC Research Notes

Figure 7

From: Characterization of the developing small intestine in the absence of either GATA4 or GATA6

Figure 7

Loss of GATA6 from the ileal epithelium alters secretory and proliferative cell populations. (A) Goblet cells, identified by MUCIN 2 (MUC2) IHC, were increased in Gata6 cKO ileum compared with control tissue (G6 CTL, Gata6loxP/+, 134 ± 8 MUC2+ cells per section; G6 cKO, Gata6loxP/-Villin-Cre, 198 ± 11 MUC2+ cells per section; n = 3 embryos/genotype, 3–4 sections/embryo). (B) Enteroendocrine cells, stained with Chromogranin A (CHGA), were present in both Gata6 cKO and control ileum. qRT-PCR was used to determine the abundance of the pan-enteroendocrine cell marker Chga and the distal enteroendocrine cell marker Pyy. Expression of both was severely decreased in GATA6 mutant ileum compared with control ileum. Epithelial cells from three control (Gata6loxP/+) and four mutant ileums (Gata6loxP/-Villin-Cre) were assayed at least three times. Gapdh was used for normalization. (C) SOX9+ cells were increased in GATA6 mutant ileum compared with control ileum (G6 CTL, Gata6loxP/+, 181 ± 4 SOX9+ cells/section; G6 cKO, Gata6loxP/-Villin-Cre, 236 ± 11 SOX9+ cells/section; n = 3 embryos/genotype, 3 sections/embryo). (D) KI67+ cells were increased in GATA6 mutant ileum compared with control ileum (G6 CTL, Gata6loxP/+, 340 ± 30 KI67+ cells/section; G6 cKO, Gata6loxP/-Villin-Cre, 421 ± 8 KI67+ cells/section; n = 3 embryos/genotype, 5–6 sections/embryo). All P-values were determined by two-sample Student’s t test: *P ≤ 0.05, **P ≤ 0.01, #P = 0.059. All error bars show SEM. Scale bars in all micrographs are 50 μm. All experiments performed using E18.5 embryos.

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