Figure 5
Melt curve kinetics for quintuplex non target species. This figure illustrates how a Brucella isolate that is not a member of one of the five major species would be excluded by this assay. The curves in grey are traces from the five species targeted by the quintuplex whilst the traces in blue represent a non-target Brucella species (in this case B. microti). The blue traces are not congruent with any of the grey traces indicting that the sample tested represents a species not included in this assay.