Figure 1
From: Experimental validation of predicted subcellular localizations of human proteins
The cartoon shows the research strategy used to experimentally validate the predicted subcellular localization of proteins. Genes of interest were cloned into pEGP-N1 vector as GFP fusions. These GFP-tagged genes along with corresponding location-specific RFP-tagged gene markers, were transiently co-transfected using liposome-mediated method into two normal breast and breast cancer cell lines. Following 24Â hours of transfection and protein expression, subcellular localization was determined using live cell imaging/confocal microscopy. Protein localizations to each compartment were confirmed by observing the colocalization of GFP- and RFP-tagged proteins that gives yellow color up on merging green and red images.