Table 1 Clinical signs of toxicity at the oral limit dose (2000 mg/kg bw)
From: Hydroxytyrosol: lack of clastogenicity in a bone marrow chromosome aberration study in rats
Observation | Time (post application) | ||||||
|---|---|---|---|---|---|---|---|
30 min | 1 hour | 2 hours | 3 hours | 4 hours | 24 hours | 48 hours | |
Number of animals | 10/sex | 10/sex | 10/sex | 10/sex | 10/sex | 10/sex | 10/sex |
Reduced spontaneous activity | 6 M, 1 F | 0 M, 1 F | 0 M, 1 F | 0 M, 1 F | 0 M, 1 F | 0 M, 0 F | 0 M, 0 F |
Ataxia | 0 M, 1 F | 0 M, 1 F | 0 M, 1 F | 0 M, 1 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F |
Wasp waist | 0 M, 1 F | 0 M, 1 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F |
Piloerection | 0 M, 1 F | 0 M, 1 F | 0 M, 1 F | 0 M, 1 F | 0 M, 1 F | 0 M, 0 F | 0 M, 0 F |
Moving the bedding | 0 M, 1 F | 0 M, 1 F | 0 M, 1 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F |
Salivation | 0 M, 1 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F |
Prone position | 0 M, 1 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F | 0 M, 0 F |