Flow chart of the comprehensive informatics approach to alternative splicing. 262 differentially alternatively spliced (DAS) exons obtained by Exon Array analysis were tested because it was suggested that approximately 87% of these DAS exons were changed during neuronal differentiation of P19 cells . The exons were determined and their transcripts with/without DAS exons were collected and translated. A conserved domain search suggested that the functional domains could be changed in 128 pairs via alternative splicing. Moreover, comparison of gene annotations and their domain descriptions suggested that alternative splicing in 49 genes affected their critical functional domain. Pathway analysis suggested that the EGFR/mTOR signaling pathway could be the most affected molecular network.