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Table 1 Comparison of the expression stability of 6 reference genes in fetal sheep liver as calculated by geNorm, Normfinder and BestKeeper

From: The dilution effect and the importance of selecting the right internal control genes for RT-qPCR: a paradigmatic approach in fetal sheep

Rank geNorm NormFinder BestKeeper BestKeeper
  Gene Stability Gene Stability Gene Stability* Gene Stability**
1 PPIA 0.50 PPIA 0.188 HPRT1 2.17 RNA18S 27.2
2 HPRT1 0.50 HPRT1 0.326 PPIA 2.43 PPIA 32.2
3 RNA18S 0.58 RPLPO 0.349 RNA18S 3.26 HPRT1 33.3
4 RPLPO 0.64 RNA18S 0.410 RPLPO 3.60 RPLPO 40.7
5 TUBB 0.71 TUBB 0.443 ACTB 3.75 TUBB 49.1
6 ACTB 0.75 ACTB 0.454 TUBB 4.45 ACTB 50.0
  1. *% coefficient of variation compared to the mean cycle at the threshold (Ct).
  2. **% coefficient of variation compared to the mean relative abundance (calculated as ECtmin-Ctsample).
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BMC Research Notes

ISSN: 1756-0500

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