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Table 1 Comparison of the expression stability of 6 reference genes in fetal sheep liver as calculated by geNorm, Normfinder and BestKeeper

From: The dilution effect and the importance of selecting the right internal control genes for RT-qPCR: a paradigmatic approach in fetal sheep

Rank

geNorm

NormFinder

BestKeeper

BestKeeper

 

Gene

Stability

Gene

Stability

Gene

Stability*

Gene

Stability**

1

PPIA

0.50

PPIA

0.188

HPRT1

2.17

RNA18S

27.2

2

HPRT1

0.50

HPRT1

0.326

PPIA

2.43

PPIA

32.2

3

RNA18S

0.58

RPLPO

0.349

RNA18S

3.26

HPRT1

33.3

4

RPLPO

0.64

RNA18S

0.410

RPLPO

3.60

RPLPO

40.7

5

TUBB

0.71

TUBB

0.443

ACTB

3.75

TUBB

49.1

6

ACTB

0.75

ACTB

0.454

TUBB

4.45

ACTB

50.0

  1. *% coefficient of variation compared to the mean cycle at the threshold (Ct).
  2. **% coefficient of variation compared to the mean relative abundance (calculated as ECtmin-Ctsample).