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Table 3 Primer information for candidate reference genes and rRNA in fetal sheep liver

From: The dilution effect and the importance of selecting the right internal control genes for RT-qPCR: a paradigmatic approach in fetal sheep

Gene symbol Primer sequences (5’ → 3’) Product size Efficiency* Accession No. Function and reference
RPLP0 F:CAA CCC TGA AGT GCT TGA CAT 227 bp 95.3% NM_001012682 Protein metabolism and modification [59,60]
R: AGG CAG ATG GAT CAG CCA
TUBB F:GGT CCT GGA TGT GGT TCG GAA G 223 bp 99.78% GQ338157 Member of a small family of globular proteins [62]
R:GAC GGA GAG GGT GGC ATT GTA G
RNA18S F: GCT ACC ACA TCC AAG GAA GG 244 bp 95.62% NR_036642.1 Recognition role, involved in correct positioning of the mRNA and tRNA [26,51]
R: GCT CCC AAG ATC CAA CTA CG
ACTB F:CAT CGG CAA TGA GCG GTT CC 146 bp 96.02% NM_001009784 Cytoskeletal structural protein [60]
R:CCG TGT TGG CGT AGA GGT
HPRT1 F:GCT GAG GAT TTG GAG AAG GTG T 94 bp 95.6% NM_001034035.1 Nucleoside, nucleotide and nucleic acid metabolism [60]
R:GGC CAC CCA TCT CCT TCA T
PPIA F:TGT GCC AGG GTG GTG ACT TCA 196 bp 100% AY251270 Protein metabolism and modification [60]
R:TGC TTG CCA TCC AAC CAC TCA G
NR3C1 F: ACT GCC CCA AGT GAA AAC AGA 151 bp 95.6% NM_001114186 glucocorticoid receptor [63]
R: ATG AAC AGA AAT GGC AGA CAT
IGF1 F: TTG GTG GAT GCT CTC CAG TTC 118 bp 95.1% NM_001009774.2 Insulin like growth factor type 1 [59]
R: AGC AGC ACT CAT CCA CGA TTC
G6PC F: GGA TTC TGG ATC GTG CAA CT 196 bp 100% EF062861.1 Glucose 6 phosphatase [61]
R: ATC CAA TGG CGA AAC TGA AC
RNA28S F: AAC TCT GGT GGA GGT CCG TAG C 115 bp 97.5% XM_004023057 structural RNA for the large component of eukaryotic cytoplasmic ribosomes, and thus one of the basic components of all eukaryotic cells
R: GAG GGA AAC TTC GGA GGG AAC C
  1. *PCR efficiencies were determined using the formula (10-1/slope-1) × 100%.
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