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Table 3 Primer information for candidate reference genes and rRNA in fetal sheep liver

From: The dilution effect and the importance of selecting the right internal control genes for RT-qPCR: a paradigmatic approach in fetal sheep

Gene symbol

Primer sequences (5’ → 3’)

Product size

Efficiency*

Accession No.

Function and reference

RPLP0

F:CAA CCC TGA AGT GCT TGA CAT

227 bp

95.3%

NM_001012682

Protein metabolism and modification [59,60]

R: AGG CAG ATG GAT CAG CCA

TUBB

F:GGT CCT GGA TGT GGT TCG GAA G

223 bp

99.78%

GQ338157

Member of a small family of globular proteins [62]

R:GAC GGA GAG GGT GGC ATT GTA G

RNA18S

F: GCT ACC ACA TCC AAG GAA GG

244 bp

95.62%

NR_036642.1

Recognition role, involved in correct positioning of the mRNA and tRNA [26,51]

R: GCT CCC AAG ATC CAA CTA CG

ACTB

F:CAT CGG CAA TGA GCG GTT CC

146 bp

96.02%

NM_001009784

Cytoskeletal structural protein [60]

R:CCG TGT TGG CGT AGA GGT

HPRT1

F:GCT GAG GAT TTG GAG AAG GTG T

94 bp

95.6%

NM_001034035.1

Nucleoside, nucleotide and nucleic acid metabolism [60]

R:GGC CAC CCA TCT CCT TCA T

PPIA

F:TGT GCC AGG GTG GTG ACT TCA

196 bp

100%

AY251270

Protein metabolism and modification [60]

R:TGC TTG CCA TCC AAC CAC TCA G

NR3C1

F: ACT GCC CCA AGT GAA AAC AGA

151 bp

95.6%

NM_001114186

glucocorticoid receptor [63]

R: ATG AAC AGA AAT GGC AGA CAT

IGF1

F: TTG GTG GAT GCT CTC CAG TTC

118 bp

95.1%

NM_001009774.2

Insulin like growth factor type 1 [59]

R: AGC AGC ACT CAT CCA CGA TTC

G6PC

F: GGA TTC TGG ATC GTG CAA CT

196 bp

100%

EF062861.1

Glucose 6 phosphatase [61]

R: ATC CAA TGG CGA AAC TGA AC

RNA28S

F: AAC TCT GGT GGA GGT CCG TAG C

115 bp

97.5%

XM_004023057

structural RNA for the large component of eukaryotic cytoplasmic ribosomes, and thus one of the basic components of all eukaryotic cells

R: GAG GGA AAC TTC GGA GGG AAC C

  1. *PCR efficiencies were determined using the formula (10-1/slope-1) × 100%.