Multiple alignment of CYP4X1 with templates. The target CYP4X1 (Q8N118) and six templates (1TQN, 3CZH, 2HI4, 3NA0, 3K9V, 3E4E) were aligned using ClustalO program. The red color indicates conserved residues and yellow colour indicates a semi-consereved substitution. The signature motifs are shown in the box. The alignment figure was prepared using ESpript 2.2.