Radioactivity accumulation after [
F]FDG labelling and simulated herbivory in
(A) Scheme of the experimental setup. Leaves of 3 week old plants were punctured with a needle and applied with FDG (red dots). Another leaf kept untreated (CON) or was wounded and treated with 1 μL of water (WW, blue dots) or 1 μL of 1:5 diluted Manduca sexta oral secretions (WOS, green dots). After 8 h the plants were disassembled and an autoradiographic picture was taken from a set of plants. Plant parts were then weighed and radioactivity was measured. (B) Autoradiographs of roots. Red arrows indicate changes at root tips. (C) Radioactivity measurements in roots and sink leaves of [18F]FDG-labelled wild type (WT) and inverted repeat COI1 (irCOI1) plants. Letters indicate significant differences between treatments (ANOVA, root: F2,12 = 5.16; P = 0.077; young leaves: F2,12 = 3.06; P = 0.0301), N ≥ 5 ± SE.