Figure 2
[ 18 F]FDG and [ 19 F]FDG metabolism in N. attenuata leaves. (A) [18F]FDG is metabolized in Nicotiana attenuata. One leaf (AP) of a 3.5 week old plant was punctured with a needle and applied with 5 μL [18F]FDG solution. Another leaf was induced with wounding and treated with 1 μL of 1:5 diluted Manduca sexta oral secretions (IL). After 8 hours the plants were disassembled, tissues were extracted and qualitative sugar analysis was done by performing thin layer chromatography (TLC, left picture). Autoradiograph was taken of the same TLC plate (right picture). Labeling: YL = youngest leaves, IL = induced leaf, RO = root, AP = apical part of the [18F]FDG treated leaf, AU = apical bud of the plant, AB = basal part of the [18F]FDG treated leaf, standards: S = sucrose, F = fructose, G = glucose, UDPG = uridindiphosphate-glucose, G6P = glucose-6-phosphate, F6P = fructose-6-phosphate, FDG = [18F]FDG. (B) Comparison of total (TIC) and extracted ion chromatograms ([19 F]FDG disaccharide: m/z 343.10) of leaf extract (ctrl, i and iii) and [19F]FDG applied leaf extract (ii and iv). (C) MS2 of m/z: 343.10 (retention time: 5.50 min). (D) Comparison TIC of CTRL-leaf extract (i) with [19F]FDG applied leaf extract (ii) for depicting [19F]FDG and [19F]-disaccharide chromatographic peaks.