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Figure 4 | BMC Research Notes

Figure 4

From: Development of Loop-Mediated Isothermal Amplification (LAMP) assay for rapid detection of Fusarium oxysporum f. sp. ciceris - wilt pathogen of chickpea

Figure 4

Comparative sensitivity of LAMP and conventional PCR analysis for detection of Fusarium oxysporum f. sp. ciceris using serial dilutions of DNA as template. A) Electrophoresis analysis of LAMP. Lane 1: 10 μg; lane 2: 1 μg; lane 3: 100 ng; lane 4: 10 ng; lane 5: 1 ng; lane 6: 100 pg; lane 7: 10 pg; lane 8: 1 pg; lane 9: 100 fg; lane 10: 10 fg. Lane M, 100 bp DNA Marker. B) Electrophoresis analysis of conventional PCR with ITS-1 and ITS-4 primers. Lane M, DNA marker (1 Kb). C) Visual detection of LAMP assay by addition of HNB.

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