|
Neutral red uptake assay
|
1. Cell enumeration independent of enzymatic conversion of dye [30,31]
|
1. Some reports of test compound interference [32]
|
|
2. Few wash steps involved [30,31]
|
|
MTT assay
|
1. Gold standard for cytotoxicity testing
|
1. Conversion to formazan crystals depends on metabolic rate and number of mitochondria resulting in many known interferences [4,12-17,33]
|
|
2. Suitable for high-throughput screening and miniaturisation [34]
|
2. Numerous wash steps required [3,7]
|
|
Resazurin reduction assay
|
1. Few wash steps involved [19]
|
1. Conversion to resorufin depend on enzymatic conversion [18]
|
|
2. Follow-up assays can be performed on same cells as assay is not cytotoxic [32,35]
|
2. Absorbance-based method less sensitive than fluorescence-based method
|
|
Sulforhodamine B assay
|
1. Cell enumeration dependent on protein content thus no test compound interference [21,22]
|
1. Numerous wash steps involved, but fixation required [22]
|
|
2. Highly reproducible
|
2. Less sensitive with non-adherent cells
|
