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Table 3 The IC 50 concentrations of 3-bromopyruvate calculated for MCF-7 cells after 24 or 72 hour incubation

From: Limitations of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays

  Cytotoxicity assay
NRU MTT RES SRB
24 hour Incubation 54.1 ± 1.6 - - 86.4 ± 1.2
72 hour incubation 60.9 ± 1.3 98.5 ± 1.1 105.9 ± 1.1 74.4 ± 1.1
  1. Where no values are given these could not be calculated as the assay reported relative cell survival of greater than 50% at the tested concentrations (n = 4).
  2. Results are indicated as mean ± SEM.
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