Skip to main content

Table 3 The IC 50 concentrations of 3-bromopyruvate calculated for MCF-7 cells after 24 or 72 hour incubation

From: Limitations of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays

 

Cytotoxicity assay

NRU

MTT

RES

SRB

24 hour Incubation

54.1 ± 1.6

-

-

86.4 ± 1.2

72 hour incubation

60.9 ± 1.3

98.5 ± 1.1

105.9 ± 1.1

74.4 ± 1.1

  1. Where no values are given these could not be calculated as the assay reported relative cell survival of greater than 50% at the tested concentrations (n = 4).
  2. Results are indicated as mean ± SEM.