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Figure 3 | BMC Research Notes

Figure 3

From: Compensatory growth in novel Drosophila Akt1 mutants

Figure 3

Novel Akt1 mutants are reduced in both ommatidia size and number, enhanced in somatic clones and partially rescued by expression of Akt1 + . Scanning electron micrographs illustrate the phenotypes of the homozygote, somatic clone and transgenic rescue of the control, original P-element insertion mutant and each of the three novel Akt1 mutants. Top row images (A-E) of homozygous mutants signify that hypomorphic alleles of Akt1 are reduced in size. The middle row images (F-J) of somatic clones of these alleles reveal an increase in severity of this phenotype. The bottom row of images (K-O) express wild type Akt1 in the background of the hypomorphic alleles and validate these phenotypes are Akt1 dependent. Full genotypes are A: w; P[FRT;w + ] 2A P[ry + neo R FRT] 82B Akt1 + /Akt1 + B: w; P[FRT;w + ] 2A P[ry + neo R FRT] 82B. Akt1 04226 /Akt1 04226 C: w; P[FRT;w + ] 2A P[ry + neo R FRT] 82B Akt1 52 /Akt1 52 D: w; P[FRT;w + ] 2A P[ry + neo R FRT] 82B Akt1 57 /Akt1 57 E: w; P[FRT; w + ] 2A P[ry + neo R FRT] 82B Akt1 87 /Akt1 87 F: y w; P{w +m= GAL4-ey.H} 3–8 P{w +mC= UAS-FLP1.D} JD1 /+; P[FRT ;w + ] 2A P[ry + neo R FRT] 82B Akt1 + /P{ry +t7.2= neoFRT} 82B P{w +mC= GMR-hid} SS4 l(3)CL-R 1 G: y w; P{w +m= GAL4-ey.H} 3–8 P{w +mC= UAS-FLP1.D} JD1 /+; P[FRT; w + ] 2A P[ry + neo R FRT]82B Akt104226/ P{ry + t7.2 = neoFRT}82B P{w + mC = GMR-hid}SS4 l(3)CL-R1 H: y w; P{w +m= GAL4-ey.H} 3–8 P{w +mC= UAS-FLP1.D} JD1 /+; P[FRT;w + ] 2A P[ry + neo R FRT] 82B Akt1 52 /P{ry +t7.2= neoFRT} 82B P{w +mC= GMR-hid} SS4 l(3)CL-R 1 I: y w; P{w +m= GAL4ey.H} 3–8 P{w +mC= UAS-FLP1.D} JD1 /+; P[FRT ;w + ] 2A P[ry + neo R FRT] 82B Akt1 57 /P{ry +t7.2= neoFRT} 82B P{w +mC= GMR-hid} SS4 l(3)CL-R 1 J: y w; P{w +m= GAL4-ey.H} 3–8 P{w +mC= UAS-FLP1.D} JD1 /+; P[FRT ;w + ] 2A P[ry + neo R FRT] 82B Akt1 87 /P{ry+t7.2 = neoFRT}82B P{w+mC = GMR-hid}SS4 l(3)CL-R1 K: w1118; UAS-Akt1+/arm-GAL4; Akt1 + /Akt1 + L: w 1118 ; UAS-Akt1 + /arm-GAL4; Akt1 04226 /Akt1 04226 M: w 1118 ; UASAkt1 + /arm-GAL4; Akt1 52 /Akt1 52 N: w 1118 ; UAS-Akt1 + /arm-GAL4; Akt1 57 /Akt1 57 O: w 1118 ; UAS-Akt1 + /arm-GAL4; Akt1 87 /Akt1 87 . Scale bar = 100 um. Biometric analysis of both the number, (P) and area, (Q) of ommatidia quantifies the subtlety of these size differences. N values can be found in Table 1. Green bars represent the homozygotes; purple bars represent the clones and blue bars represent the transgenic rescues. The original P-element insertion mutant Akt1 04226 is smaller than the control but larger than the novel mutants in both size and number. All three novel Akt1 mutants are reduced in both the area and number of ommatidia as both homozygotes or clones but reach almost comparable measurements compared to the control when rescued by a transgene. Error bars represent standard error of the mean (p = <0.05). Further statistical analysis can be found in Table 1.

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