Experimental design setup and representative DAPK Western blots. A: Subset design to analyze the effects of simultaneous shear stress and stimuli. Cells are treated to shear stress either before or after treatment with apoptotic stimuli, which includes TNFα, H2O2, and serum depletion. B: Western blot of DAPK in pre-sheared cells versus cells treated with stimulus (TNFα, H2O2, or serum depletion). C: Western blot of DAPK in cells sheared post treatment with stimulus (TNFα, H2O2, or serum depletion).