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Figure 1 | BMC Research Notes

Figure 1

From: Cyclooxygenase 1 mRNA expression is undetectable in Madin Darby Canine Kidney cells

Figure 1

Expression of cyclooxygenases in MDCK cells and dog kidney. A) Schematic representation of the exonic structure of the PTGS1 transcript (coding for the COX-1 protein) in dog, mouse, rat and human. Exon structure and alignment were based on NCBI RefSeq NM_001003023, NM_08969, NM_017043 and NM_001271162, accessed on February 6, 2015. Exons are numbered and their lengths indicated along with the locations of the PCR primers used in this study. Exons are not all shown to scale. B) Representative gel showing the expression of COX-1 (PTGS1), COX-2 (PTGS2) and HPRT1 in MDCK cells (M) and dog kidney (K). Three regions of the PTGS1 gene, namely Exon 10-11 (E10-11), E8-9 and E4-5 were targeted for PCR amplification. The Cox1_E4-5 primers were also used to amplify genomic DNA from MDCK cells and dog kidney, generating a larger amplicon (380 bp) due to the inclusion of a 212 bp intron. A 50-bp DNA ladder (L) is shown on the left.

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