From: Cyclooxygenase 1 mRNA expression is undetectable in Madin Darby Canine Kidney cells
Reference | Summary of findings |
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Yang et al. [2] | Western blot: COX-2 observed in control MDCK cells and strongly induced by hyperosmolarity. COX-1 expression was not detected in MDCK cells. |
Schaeffers et al. [11] | Western blot: COX-1 and COX-2 not detected in MDCK cells. |
Northern blot (mouse probe): COX-1 and COX-2 not detected in MDCK cells. | |
RT-PCR (based on consensus sequence in mouse, rat and man): Lowly expressed COX-2 in control MDCK cells is induced by TPA exposure. COX-1 expression not detected in control and TPA-treated MDCK cells. | |
Sciorra and Daniel [12] | Western blot: Low COX-1 and COX-2 levels in Control MDCK cells and strong COX-2 (but not COX-1) induction following TPA exposure. |
Ostrom et al. [13] | Western blot: COX-1 and COX-2 expressed in MDCK cells, but not induced by a variety of factors, including TPA. |
RT-PCR (based on conserved sequence across species): COX-2 transcript amplified, but not COX-1 (despite numerous attempts). | |
Benitah et al. [14] | Western blot: COX-1 and COX-2 both detected in control MDCK cells. |
Cowley et al. [15] | Northern blot (mouse probe): COX-2 transcript present in control MDCK cells and quickly induced by hyperosmolarity. COX-1 not detected in control MDCK cells but weak signal detected following chronic hyperosmolarity. (Cross-hybridisation of cyclooxygenase probes was observed.) |
Kay-Mugford et al. [16] | Northern blot (human probe): COX-1 and COX-2 detected in MDCK cells. |
RT-PCR (primers based on human sequence): COX-2 expression not detected in MDCK cells. | |
Knottenbelt et al. [17] | Western blot: COX-1 and COX-2 not detected in MDCK cells. |
Reyes-Martin et al. [18] | Western blot: COX-1 and COX-2 both detected in control MDCK cells and strongly induced following H2O2 exposure. |
Flores-Benitez et al. [19] | Western blot: COX-1 and COX-2 both detected in control MDCK cells, but not induced following EGF treatment. |
Steinert et al. [20] | Western blot: COX-2 expressed in control MDCK cells and induced by hyperosmolarity. |
Northern blot (human probe): COX-2 expressed in control MDCK cells and induced by hyperosmolarity. |