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Table 1 Summary of cited studies on the expression of COX-1 and COX-2 in MDCK cells

From: Cyclooxygenase 1 mRNA expression is undetectable in Madin Darby Canine Kidney cells

Reference Summary of findings
Yang et al. [2] Western blot: COX-2 observed in control MDCK cells and strongly induced by hyperosmolarity. COX-1 expression was not detected in MDCK cells.
Schaeffers et al. [11] Western blot: COX-1 and COX-2 not detected in MDCK cells.
Northern blot (mouse probe): COX-1 and COX-2 not detected in MDCK cells.
RT-PCR (based on consensus sequence in mouse, rat and man): Lowly expressed COX-2 in control MDCK cells is induced by TPA exposure. COX-1 expression not detected in control and TPA-treated MDCK cells.
Sciorra and Daniel [12] Western blot: Low COX-1 and COX-2 levels in Control MDCK cells and strong COX-2 (but not COX-1) induction following TPA exposure.
Ostrom et al. [13] Western blot: COX-1 and COX-2 expressed in MDCK cells, but not induced by a variety of factors, including TPA.
RT-PCR (based on conserved sequence across species): COX-2 transcript amplified, but not COX-1 (despite numerous attempts).
Benitah et al. [14] Western blot: COX-1 and COX-2 both detected in control MDCK cells.
Cowley et al. [15] Northern blot (mouse probe): COX-2 transcript present in control MDCK cells and quickly induced by hyperosmolarity. COX-1 not detected in control MDCK cells but weak signal detected following chronic hyperosmolarity. (Cross-hybridisation of cyclooxygenase probes was observed.)
Kay-Mugford et al. [16] Northern blot (human probe): COX-1 and COX-2 detected in MDCK cells.
RT-PCR (primers based on human sequence): COX-2 expression not detected in MDCK cells.
Knottenbelt et al. [17] Western blot: COX-1 and COX-2 not detected in MDCK cells.
Reyes-Martin et al. [18] Western blot: COX-1 and COX-2 both detected in control MDCK cells and strongly induced following H2O2 exposure.
Flores-Benitez et al. [19] Western blot: COX-1 and COX-2 both detected in control MDCK cells, but not induced following EGF treatment.
Steinert et al. [20] Western blot: COX-2 expressed in control MDCK cells and induced by hyperosmolarity.
Northern blot (human probe): COX-2 expressed in control MDCK cells and induced by hyperosmolarity.