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Table 1 Summary of cited studies on the expression of COX-1 and COX-2 in MDCK cells

From: Cyclooxygenase 1 mRNA expression is undetectable in Madin Darby Canine Kidney cells

Reference

Summary of findings

Yang et al. [2]

Western blot: COX-2 observed in control MDCK cells and strongly induced by hyperosmolarity. COX-1 expression was not detected in MDCK cells.

Schaeffers et al. [11]

Western blot: COX-1 and COX-2 not detected in MDCK cells.

Northern blot (mouse probe): COX-1 and COX-2 not detected in MDCK cells.

RT-PCR (based on consensus sequence in mouse, rat and man): Lowly expressed COX-2 in control MDCK cells is induced by TPA exposure. COX-1 expression not detected in control and TPA-treated MDCK cells.

Sciorra and Daniel [12]

Western blot: Low COX-1 and COX-2 levels in Control MDCK cells and strong COX-2 (but not COX-1) induction following TPA exposure.

Ostrom et al. [13]

Western blot: COX-1 and COX-2 expressed in MDCK cells, but not induced by a variety of factors, including TPA.

RT-PCR (based on conserved sequence across species): COX-2 transcript amplified, but not COX-1 (despite numerous attempts).

Benitah et al. [14]

Western blot: COX-1 and COX-2 both detected in control MDCK cells.

Cowley et al. [15]

Northern blot (mouse probe): COX-2 transcript present in control MDCK cells and quickly induced by hyperosmolarity. COX-1 not detected in control MDCK cells but weak signal detected following chronic hyperosmolarity. (Cross-hybridisation of cyclooxygenase probes was observed.)

Kay-Mugford et al. [16]

Northern blot (human probe): COX-1 and COX-2 detected in MDCK cells.

RT-PCR (primers based on human sequence): COX-2 expression not detected in MDCK cells.

Knottenbelt et al. [17]

Western blot: COX-1 and COX-2 not detected in MDCK cells.

Reyes-Martin et al. [18]

Western blot: COX-1 and COX-2 both detected in control MDCK cells and strongly induced following H2O2 exposure.

Flores-Benitez et al. [19]

Western blot: COX-1 and COX-2 both detected in control MDCK cells, but not induced following EGF treatment.

Steinert et al. [20]

Western blot: COX-2 expressed in control MDCK cells and induced by hyperosmolarity.

Northern blot (human probe): COX-2 expressed in control MDCK cells and induced by hyperosmolarity.