Table 2 Summary of number of molecules identified with different methylation patterns in IP assays using 5-methyl-C antibody with input DNA of mixed methylation patterns
From: Sensitivity and specificity of immunoprecipitation of DNA containing 5-Methylcytosine
Region | Methylation status | #CpG in | # of molecules detected in | % of total | ||
|---|---|---|---|---|---|---|
MseI frag. | Amplicon | Exp 1 | Exp 2 | |||
1 | Unmethylated | 0 | 0 | 7 | 7 | 24.6 |
Hha-methylated | 2 | 0 | 0 | 0 | ||
HpaII-methylated | 11 | 2 | 4 | 8 | 21.1 | |
Fully methylated | 54 | 11 | 17 | 14 | 54.4 | |
2 | Unmethylated | 0 | 0 | 3 | 4 | 11.3 |
Hha-methylated | 0 | 0 | 0 | 0 | ||
HpaII-methylated | 2 | 1 | 0 | 3 | 4.8 | |
Fully methylated | 12 | 11 | 27 | 25 | 83.9 | |
3 | unmethylated | 0 | 0 | 2 | 5 | 10.3 |
Hha-methylated | 3 | 2 | 1 | 2 | 2.2 | |
HpaII-methylated | 3 | 1 | 1 | 5 | 8.8 | |
Fully methylated | 23 | 17 | 25 | 27 | 76.5 | |