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Table 2 Summary of number of molecules identified with different methylation patterns in IP assays using 5-methyl-C antibody with input DNA of mixed methylation patterns

From: Sensitivity and specificity of immunoprecipitation of DNA containing 5-Methylcytosine

Region Methylation status #CpG in # of molecules detected in % of total
MseI frag. Amplicon Exp 1 Exp 2
1 Unmethylated 0 0 7 7 24.6
Hha-methylated 2 0 0 0
HpaII-methylated 11 2 4 8 21.1
Fully methylated 54 11 17 14 54.4
2 Unmethylated 0 0 3 4 11.3
Hha-methylated 0 0 0 0
HpaII-methylated 2 1 0 3 4.8
Fully methylated 12 11 27 25 83.9
3 unmethylated 0 0 2 5 10.3
Hha-methylated 3 2 1 2 2.2
HpaII-methylated 3 1 1 5 8.8
Fully methylated 23 17 25 27 76.5