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Table 3 Summary of number of molecules identified with different methylation patterns in IP assays using anti-MBD2 antibody with input DNA of mixed methylation patterns

From: Sensitivity and specificity of immunoprecipitation of DNA containing 5-Methylcytosine

Region Methylation status # CpG in # of molecules detected % of total
MseI frag. Ampl-icon Exp 1 Exp 2 Exp 3 Exp 4
1 unmethylated 0 0 1 0    3.0
Hha-methylated 2 0 0 0  
HpaII-methylated 11 2 1 0    3.0
Fully methylated 54 11 15 16    93.9
2 unmethylated 0 0 1 1    11.8
Hha-methylated 0 0 0 0  
HpaII-methylated 2 1 1 0    5.9
Fully methylated 12 11 6 8    82.4
3 unmethylated 0 0 15 14 7 5 80.4
Hha-methylated 3 2 0 0 0 0 0
HpaII-methylated 3 1 0 0 0 0 0
Fully methylated 23 17 0 6 1 3 19.6