Table 3 Summary of number of molecules identified with different methylation patterns in IP assays using anti-MBD2 antibody with input DNA of mixed methylation patterns
From: Sensitivity and specificity of immunoprecipitation of DNA containing 5-Methylcytosine
Region | Methylation status | # CpG in | # of molecules detected | % of total | ||||
|---|---|---|---|---|---|---|---|---|
MseI frag. | Ampl-icon | Exp 1 | Exp 2 | Exp 3 | Exp 4 | |||
1 | unmethylated | 0 | 0 | 1 | 0 | Â | Â | 3.0 |
Hha-methylated | 2 | 0 | 0 | 0 | Â | |||
HpaII-methylated | 11 | 2 | 1 | 0 | Â | Â | 3.0 | |
Fully methylated | 54 | 11 | 15 | 16 | Â | Â | 93.9 | |
2 | unmethylated | 0 | 0 | 1 | 1 | Â | Â | 11.8 |
Hha-methylated | 0 | 0 | 0 | 0 | Â | |||
HpaII-methylated | 2 | 1 | 1 | 0 | Â | Â | 5.9 | |
Fully methylated | 12 | 11 | 6 | 8 | Â | Â | 82.4 | |
3 | unmethylated | 0 | 0 | 15 | 14 | 7 | 5 | 80.4 |
Hha-methylated | 3 | 2 | 0 | 0 | 0 | 0 | 0 | |
HpaII-methylated | 3 | 1 | 0 | 0 | 0 | 0 | 0 | |
Fully methylated | 23 | 17 | 0 | 6 | 1 | 3 | 19.6 | |