Figure 2

Analysed targets represent a wide range of quantities. The quantification cycle (Cq), which is inversely proportional to the amount of target nucleic acid in the sample, was used to calculate the approximate amount of the targets. RT-qPCR analysis was performed with the untreated RNA isolated from three independent mice hearts (one male - grey, and two female - black). (A) Data-points and the mean of the Cq values of Nppa (cross), Nppb (downward-pointing triangle), Hprt (open circle) and Polr2a (hexagon) with the SD of three independent experiments are illustrated. (B) Data-points and the mean of the Cq values of Sno202 (closed circle), miR-1 (asterisk), miR-208 (rhombus) and miR-501 (upwards-pointing triangle) with the SD of three independent experiments are illustrated. (C) Data-points and the mean of the ΔCq values of Nppa (cross), Nppb (downward-pointing triangle), Hprt (open circle) and Polr2a (hexagon) with the SD of three independent experiments after normalization with the reference mRNA Polr2. (D) Data-points and the mean of the ΔCq values of Sno202 (upwards-pointing triangle), miR-1 (closed circle), miR-208 (asterisk) and miR-501 (rhombus) with the SD of three independent experiments after normalization with the reference small RNA sno202.