Figure 3

mRNA and miRNA stabilities measured by RT-qPCR are differently influenced depending on the treatment. Percentage of intact mRNA (blue) and miRNA (black) after indicated treatments are illustrated for three independent experiments. Nppa, Nppb, Hprt, and Polr2a were each analysed as targets representative for mRNA and sno202, miR1, miR208, and miR501 each as targets for miRNA. The mean with the SD for all analysed targets of each RNA class is given in violet for mRNA and in red for miRNA. The green dotted line represents the 100% intact RNA obtained from the untreated RNA. The ordinate indicates the relative percentage of intact RNA in comparison to the untreated RNA. Significances were tested by the two tailed unpaired Student’s t-test: * = p < 0.05; ** = p < 0.01; *** = p < 0.001. ExoT₇ = Exonuclease T₇; ExoT = Exonuclease T.