Figure 2
From: Potential complications when developing gene deletion clones in Xylella fastidiosa
Confirmation of Xylella fastidiosa pilJ gene deletion. The pilJA/pilJD (AD) primers amplify a 3082 bp fragment from wild-type control cells (wt) or a 2200 bp fragment form the XfΔpilJ mutant (J) and deletion plasmid (P). The pilJE/pilJF (EF) primers amplify a 2030 bp band for the wild-type control strain and no band for the mutant cells or deletion plasmid. RST31/33 (RST) primers confirm that the bacteria were X. fastidiosa.