Figure 3From: Potential complications when developing gene deletion clones in Xylella fastidiosa Mixture of wild-type and mutant Xylella fastidiosa strains after first isolation. The XfΔpilJ mutant confirmed in Figure 2 was stored at -80°C, streaked onto periwinkle agar plates amended with kanamycin, and the genotype assessed for 12 single colonies. Each number denotes a single colony. The pilJE/pilJF (EF) primers amplified a 2030 bp band for non-transformed X. fastidiosa and no band for the XfΔpilJ mutant. Wild-type X. fastidiosa DNA (wt) was used as a positive control for the PCR reaction, while primer reaction without template DNA represented by H2O, was used as a negative control.Back to article page