Figure 7
From: Potential complications when developing gene deletion clones in Xylella fastidiosa
Protection of wild-type Xylella fastidiosa from antibiotic selection pressure. Wild-type X. fastidiosa (wt), XfΔpilJ mutant (J), or an equal mixture of both (M) were grown in PD2 liquid media before being plated onto agar plates with 0, 4, 10, 25, or 50 μg/mL kanamycin, and tested by PCR. The pilJA/pilJD (AD) primers amplified a 3082 bp band for wild-type cells and a 2200 bp fragment from the XfΔpilJ strain. The pilJE/pilJF (EF) primers amplified a 2030 bp band for wild-type bacteria and no equivalent bands for the XfΔpilJ mutant strains. For mixed samples, the AD primers amplified a Xf pilJ strain fragment and the EF primers amplified a wild-type band. The RST31/33 (RST) primers confirmed that the bacteria were X. fastidiosa. Wells of each cell type and kanamycin concentration condition are numbered as follows: (1) AD amplification, (2) EF amplification, and (3) RST amplification.