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Table 1 Spectrophotometric determination of total RNA quantity and quality

From: Broad application of a simple and affordable protocol for isolating plant RNA

 

TRI reagent

Oñate-Sánchez and Vicente-Carbajosa, 2008

Samples

Concentration (ng/μL)

A260/280

A260/230

Concentration (ng/μL)

A260/280

A260/230

Arabidopsis 1

355.2

2.12

1.91

613.3

2.11

2.36

Arabidopsis 2

497.4

2.00

1.90

501.6

2.03

2.19

Arabidopsis 3

231.7

2.05

1.65

481.3

2.10

2.18

Tomato 1

404.7

2.11

2.06

286.0

2.00

2.07

Tomato 2

382.9

1.99

1.88

458.3

2.12

2.09

Tomato 3

340.0

2.11

1.95

592.3

2.07

2.16

Wheat 1

238.0

2.07

1.51

379.1

2.08

2.20

Wheat 2

341.5

2.12

1.40

581.1

2.15

2.33

Wheat 3

332.5

2.10

2.02

340.0

2.07

2.24

  1. RNA concentration and absorbance ratios (A260/A230 and A260/A280) are indicated per sample and extraction method. Samples were extracted and analysed in triplicates, as indicated. Starting material per sample was as follows: Arabidopsis leaves – eight leaf discs (7 mm diameter) of five-week-old plants grown in short day conditions; wheat leaves – eight leaf discs (7 mm diameter) of four-week-old plants grown in the glasshouse; tomato leaves – eight leaf discs (7 mm diameter) of five-week-old plants grown in the glasshouse. Samples were resuspended in 30 μL of water in all cases.
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BMC Research Notes

ISSN: 1756-0500

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