Skip to main content

Table 1 Spectrophotometric determination of total RNA quantity and quality

From: Broad application of a simple and affordable protocol for isolating plant RNA

  TRI reagent Oñate-Sánchez and Vicente-Carbajosa, 2008
Samples Concentration (ng/μL) A260/280 A260/230 Concentration (ng/μL) A260/280 A260/230
Arabidopsis 1 355.2 2.12 1.91 613.3 2.11 2.36
Arabidopsis 2 497.4 2.00 1.90 501.6 2.03 2.19
Arabidopsis 3 231.7 2.05 1.65 481.3 2.10 2.18
Tomato 1 404.7 2.11 2.06 286.0 2.00 2.07
Tomato 2 382.9 1.99 1.88 458.3 2.12 2.09
Tomato 3 340.0 2.11 1.95 592.3 2.07 2.16
Wheat 1 238.0 2.07 1.51 379.1 2.08 2.20
Wheat 2 341.5 2.12 1.40 581.1 2.15 2.33
Wheat 3 332.5 2.10 2.02 340.0 2.07 2.24
  1. RNA concentration and absorbance ratios (A260/A230 and A260/A280) are indicated per sample and extraction method. Samples were extracted and analysed in triplicates, as indicated. Starting material per sample was as follows: Arabidopsis leaves – eight leaf discs (7 mm diameter) of five-week-old plants grown in short day conditions; wheat leaves – eight leaf discs (7 mm diameter) of four-week-old plants grown in the glasshouse; tomato leaves – eight leaf discs (7 mm diameter) of five-week-old plants grown in the glasshouse. Samples were resuspended in 30 μL of water in all cases.