Characterisation and expression analysis of cathepsins and ubiquitin-proteasome genes in gilthead sea bream (Sparus aurata) skeletal muscle

Table 1 Primer sequences for cloning

Primers ID	Primer sequences (5’-3’)	GenBank	Ta (°C)	Amplicon (bp)
SaCTSDb_FW	TCGGACTGTTACGATGAGGA	FM146030	56	1240
SaCTSDb_RV	CTTTGCACTTGGACGAGTTG	FG26194	56	1240
SaCTSB_FW	CCCGAAGATTATAACCAAGTTGAC	HS985610	59	1249
SaCTSB_RV	GTGACTTGTGCTCAGAAACGTAGT	FG26781	59	1249
SaUb_FW	CGGAAGTAAGAGGAACCAACAC	AM955423	56	1132
SaUb_RV	AAGCAGTCAGAATGCAAAGTCA	AM955423	56	1132
SaN3_FW	CAGGTTTGAAGCTGAGTTTCTG	HS988518	58	759
SaN3_RV	CTGACCATGTGAGCGATGTC	HS988518	58	759

Primer sequences used to clone the cathepsins (SaCTSDb and SaCTSB) and ubiquitin-proteasome members (SaUb and SaN3) from gilthead sea bream (Sa) fast muscle. FW: forward, RV: reverse; Ta: annealing temperature; bp: base pair.

ISSN: 1756-0500