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Table 2 Primer sequences for qualitative and quantitative PCR

From: Characterisation and expression analysis of cathepsins and ubiquitin-proteasome genes in gilthead sea bream (Sparus aurata) skeletal muscle

Primers ID

Primer sequences (5’-3’)

GenBank

Ta (°C)

Amplicon (bp)

SaCTSDa_qFW

CCTCCATTCACTGCTCCTTC

AF036319

56

107

SaCTSDa_qRV

ACCGGATGGAAAACTCTGTG

SaCTSDb_qFW

AAATTCCGTTCCATCAGACG

KJ524456

56

131

SaCTSDb_qRV

CTTCAGGGTTTCTGGAGTGG

SaCTSL_qFW

ACTCCTTGGGCAAACACA

DQ875329

54

116

SaCTSL_qRV

CCTTGAACTTCCTCTCCGT

SaCTSB_qFW

GCAGCCTTCCTGTTATTGG

KJ524457

57

185

SaCTSB_qRV

AGGTCCCTTCAGCATCGTA

SaUb_qFW

ACTGGCAAGACCATTACCTT

KJ524459

54

160

SaUb_qRV

TGGATGTTGTAGTCGGAAAG

SaN3_qFW

AGACACACACTGAACCCGA

KJ524458

54

118

SaN3_qRV

TTCCTGAAGCGAACCAGA

SaMuRF1_qFW

GTGACGGCGAGGATGTGC

FM145056

60

50

SaMuRF1_qRV

CTTCGGCTCCTTGGTGTCTT

SaMAFbx_qFW [2]

GGTCACCTGGAGTGGAAGAA

ERA047531

60

158

SaMAFbx_qRV [2]

GGTGCAACTTTCTGGGTTGT

Saβ-actin_qFW [44]

TCCTGCGGAATCCATGAGA

X89920

60

50

Saβ-actin_qRV [44]

GACGTCGCACTTCATGATGCT

Sa18S_qFW(a) [82]

CTCAACACGGGAAACCTCACC

NR_003286

56

119

Sa18S_qRV(a) [82]

CAGACAAATCGCTCCACCAACTA

Sa18S_qFW(b) [83]

TGACGGAAGGGCACCACCAG

AY550956

60

158

Sa18S_qRV(b) [83]

AATCGCTCCACCAACTAAGAACGG

SaEF1α_qFW [44]

CTTCAACGCTCAGGTCATCAT

AF184170

60

263

SaEF1α_qRV [44]

GCACAGCGAAACGACCAAGGGGA

  1. Primer sequences for gilthead sea bream (Sa) cathepsins (SaCTSDa, SaCTSDb, SaCTSL and SaCTSB), ubiquitin-proteasome members (SaUb, SaN3, SaMuRF1 and SaMAFbx) and reference genes (Saβ-actin, Sa18S and SaEF1α) used for conventional PCR and qPCR analyses. qFW: forward, qRV: reverse; Ta: annealing temperature; bp: base pair. (a) Sa18S primers used in the life-history stages and (b) the tissue screening experiments.