Figure 2

Validation of DE genes in the P53 pathway. The P53 pathway genes validated as differentially expressed by quantitative real-time PCR (p < 0.05). Transcripts BRCA1, BUB1, CEBPA, and CD58 were found to be differentially expressed using the ddCT method, while VDR was differentially expressed when comparing the natural log (ln) starting transcript copy number between genotypes. Fold changes in gene expression are presented, where the genotype with the lowest expression level was set to one to facilitate visualization of fold change differences.