The WT-GASP community on solid agar plates. a Colonies, inoculated with 1:1 mixture of WT/GASP bacteria (left bottom and right top), growing on solid plates show a typical sectoring pattern, as indicated by the red or green fluorescent proteins they express. Controls, WT/WT (left top) and GASP/GASP (right bottom) have a red fraction of 0.44 ± 0.12 (n = 6, mean ± standard deviation) and show that the fluorescent reporters are neutral. All scale bars indicate 3 mm. The bottom panel shows an enlarged view of the area depicted by the white square on the GASP-WT plate, the advancing edge of the colony is represented by a dashed line. b The area in between the dashed lines on the plate image is converted to polar coordinates depicting space vertically and time horizontally. The time evolution of the GASP fraction can be easily obtained from the converted image. c Traces showing the GASP fraction during the sectored growth phase. Solid lines show the means of GASP fractions for colonies inoculated with GASP fractions of 0.1 (blue
n = 6), 0.5 (red
n = 6), and 0.9 (black
n = 2); shaded areas indicate the standard deviation. GASP fractions at t = 0 show the inoculated fractions. The GASP fraction increases during formation of the homeland, while the GASP fraction remains constant in the subsequent radial expansion which typically starts after ~1 day.