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Table 1 PCR primer sequences

From: Optimizing a PCR protocol for cpn60-based microbiome profiling of samples variously contaminated with host genomic DNA

Target Primer Sequence (5′–3′)a Annealing temp (°C) Reference
cpn60 UT H279 GAIIIIGCIGGIGAYGGIACIACIAC 42, 48, 54, 60 [15]
H280 YKIYKITCICCRAAICCIGGIGCYTT
H1612 GAIIIIGCIGGYGACGGYACSACSAC
H1613 CGRCGRTCRCCGAAGCCSGGIGCCTT
16S rRNA gene SRV3-1 CGGYCCAGACTCCTAC 62 [18]
SRV3-2 TTACCGCGGCTGCTGGCAC
Pig α-actin gene JH0462 CCCAGAGCAAGCGAGGTATT 68 This study
JH0463 GGGCCTCAGTGAGCAGAGTA
  1. aI = inosine, Y = G or T, K = G or T, R = A or G, S = G or C.