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Table 1 Primers and specific probes used in the real-time multiplex PCR assay for the detection of Brucella spp., B. abortus, and B. melitensis

From: Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR

PCR Identification Primer and probe
Brucella spp. Forward primer 5′–3′ GCT-CGG-TTG-CCA-ATA-TCA-ATG-C
Reverse primer 5′–3′ GGG-TAA-AGC-GTC-GCC-AGA-AG
Probe 5′–3′ 6FAM-AAA-TCT-TCC-ACC-TTG-CCC-TTG-CCA-TCA-BHQ1
B. abortus Forward primer 5′–3′ GCG-GCT-TTT-CTA-TCA-CGG-TAT-TC
Reverse primer 5′–3′ CAT-GCG-CTA-TGA-TCT-GGT-TAC-G
Probe 5′–3′ HEX-CGC-TCA-TGC-TCG-CCA-GAC-TTC-AAT-G-BHQ1
B. melitensis Forward primer 5′–3′ AAC-AAG-CGG-CAC-CCC-TAA-AA
Reverse primer 5′–3′ CAT-GCG-CTA-TGA-TCT-GGT-TAC-G
Probe 5′–3′ Cy5-CAG-GAG-TGT-TTC-GGC-TCA-GAA-TAA-TCC-ACA-BHQ2