Table 1 Primers and specific probes used in the real-time multiplex PCR assay for the detection of Brucella spp., B. abortus, and B. melitensis
From: Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR
PCR Identification | Primer and probe | |
|---|---|---|
Brucella spp. | Forward primer 5′–3′ | GCT-CGG-TTG-CCA-ATA-TCA-ATG-C |
Reverse primer 5′–3′ | GGG-TAA-AGC-GTC-GCC-AGA-AG | |
Probe 5′–3′ | 6FAM-AAA-TCT-TCC-ACC-TTG-CCC-TTG-CCA-TCA-BHQ1 | |
B. abortus | Forward primer 5′–3′ | GCG-GCT-TTT-CTA-TCA-CGG-TAT-TC |
Reverse primer 5′–3′ | CAT-GCG-CTA-TGA-TCT-GGT-TAC-G | |
Probe 5′–3′ | HEX-CGC-TCA-TGC-TCG-CCA-GAC-TTC-AAT-G-BHQ1 | |
B. melitensis | Forward primer 5′–3′ | AAC-AAG-CGG-CAC-CCC-TAA-AA |
Reverse primer 5′–3′ | CAT-GCG-CTA-TGA-TCT-GGT-TAC-G | |
Probe 5′–3′ | Cy5-CAG-GAG-TGT-TTC-GGC-TCA-GAA-TAA-TCC-ACA-BHQ2 | |