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Table 2 Serology and real-time PCR results of serum samples collected from animals, which had aborted recently and positive in at least one test

From: Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR

Case no. Host History of samples collection after abortion (weeks) Serological assay PCRd
RBTa CFTb ELISAc Bcsp 31 IS711 B. abortus IS711 B. melitensis
1 Cow 4 + + + + +
2 Cow 3 + + + + +
3 Cow 6 + + + + +
4 Cow 4 + + + + +
5 Cow 3 + + + + +
6 Cow 3 + + + + +
7 Cow 6 + + + + +
8 Cow 4 + + + + +
9 Cow 4 + + + + +
10 Cow 3 + + + + +
11 Buffalo 4 + + + + +
12 Buffalo 1 + +
13 Buffalo 1 + +
14 Buffalo 1 + +
15 Buffalo 1 + +
16 Goat 4 + + + + +
17 Goat 3 + + + + +
18 Goat 4 + + + + +
19 Goat 1 + +
20 Goat 1 + +
21 Goat 1 + +
22 Goat 1 + +
23 Goat 1 + +
24 Goat 1 + +
25 Sheep 4 + + + + + +
Total positive 15 15 15 25 25 1
  1. aConsidered positive when showing any degree of agglutination.
  2. bPositive samples (≥20 IU/ml).
  3. cpositive samples showing cut off values (≥2).
  4. dPositive samples showing ct value (ct ≤ 40).