M-PCR improved detection of the mecA gene in vitro. To compare the detection sensitivity of the M-PCR method with the conventional real-time PCR method, we created a dilution series using cultured MRSA (×1:1 time; ×10:10 times; ×100:100 times). DNA was purified by conventional methods (A) or following centrifugation (B: M-PCR). Centrifugation promoted more rapid arrival at the threshold cycle (Fluorescence: 0.56). A comparative Ct (ΔCt) analysis was performed to examine fold changes of the mecA gene. The experiment was performed in triplicate with similar results.