Expression of the reporter gene GFP in transgenic C. richardii. GFP signal is seen in both gametophyte (b–d) and sporophyte (e–i). In T0 gametophyte, GFP signal is present in most of the cells of the prothallus (b), and strong GFP signal is seen at the antheridia (c, white arrows), and in T1 gametophytes, strongest in the sperm cells (d, white arrows) but not in the non-transformed gametophyte (control, a). In T1 sporophytes, GFP signal is present in all cells of the leaf (f), but not the control (e). GFP signal is also seen in root tissue (h) and root hair (i), but not in the root of the non-transformed sporophyte (g). Bars 1 mm.