Figure 4

Number of transgenic gametophytes regenerated from the hermaphrodites after co-incubation with Agrobacteria. Only eight gametophytes transformed with an empty vector survived selection, compared to 176 survivors transformed with the 35S::GFP construct. The majority of gametophytes transformed with 35S::GFP vector (85 gametophytes) gave rise to four to six transgenic gametophytes, 33 of them bore seven to ten gametophytes, and the rest (58 gametophytes) had at least one gametophyte. The total gametophytes used for this analysis were 21,313 and 38,364 for empty vector and 35S::GFP vector, respectively.