From: Transient and stable transformation of Ceratopteris richardii gametophytes
Enzyme concentration (w/v) with 2Â h incubation | Total samples | Number of samples showing GUS signal | GUS positive samples/total | |
---|---|---|---|---|
Cellulase | Macerozyme | |||
0.1% | 0 | 24 | 1 | 4.2% |
0.5% | 0 | 8 | 2 | 25% |
1% | 0 | 20 | 12 | 60% |
1.5% | 0 | 25 | 14 | 56% |
0.1% | 0.25% | 25 | 12 | 48% |
0.5% | 0.25% | 8 | 3 | 37.5% |
1% | 0.25% | 20 | 9 | 45% |
1.5% | 0.25% | 8 | 3 | 37.5% |
0.1% | 0.5% | 25 | 17 | 68% |
1% | 0.5% | 7 | 3 | 43% |
1.5% | 0.5% | 15 | 13 | 87% |
0 | 0.25% | 17 | 3 | 17.6% |
0 | 0.5% | 12 | 0 | 0% |
0 | 0 | 16 | 1 | 6.25% |