Skip to main content

Table 1 Effects of enzyme treatment on the success of transient transformation

From: Transient and stable transformation of Ceratopteris richardii gametophytes

Enzyme concentration (w/v) with 2 h incubation Total samples Number of samples showing GUS signal GUS positive samples/total
Cellulase Macerozyme
0.1% 0 24 1 4.2%
0.5% 0 8 2 25%
1% 0 20 12 60%
1.5% 0 25 14 56%
0.1% 0.25% 25 12 48%
0.5% 0.25% 8 3 37.5%
1% 0.25% 20 9 45%
1.5% 0.25% 8 3 37.5%
0.1% 0.5% 25 17 68%
1% 0.5% 7 3 43%
1.5% 0.5% 15 13 87%
0 0.25% 17 3 17.6%
0 0.5% 12 0 0%
0 0 16 1 6.25%