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Table 1 Effects of enzyme treatment on the success of transient transformation

From: Transient and stable transformation of Ceratopteris richardii gametophytes

Enzyme concentration (w/v) with 2 h incubation

Total samples

Number of samples showing GUS signal

GUS positive samples/total

Cellulase

Macerozyme

0.1%

0

24

1

4.2%

0.5%

0

8

2

25%

1%

0

20

12

60%

1.5%

0

25

14

56%

0.1%

0.25%

25

12

48%

0.5%

0.25%

8

3

37.5%

1%

0.25%

20

9

45%

1.5%

0.25%

8

3

37.5%

0.1%

0.5%

25

17

68%

1%

0.5%

7

3

43%

1.5%

0.5%

15

13

87%

0

0.25%

17

3

17.6%

0

0.5%

12

0

0%

0

0

16

1

6.25%