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Figure 2 | BMC Research Notes

Figure 2

From: Application of NeuroTrace staining in the fresh frozen brain samples to laser microdissection combined with quantitative RT-PCR analysis

Figure 2

Transcript levels of a 18S rRNA, b GAPDH, c BDNF, and d Arc in samples that were reverse transcribed on Day 1 (n = 6 each) and Day 8 (n = 7 each) after the ethanol and NeuroTrace (EtOH/NT) treatment, or unfixed and unstained (Untreated). The collected DG samples were 150,000 μm2 × 20 μm in volume per sample. Values are expressed as copy number of transcripts. Bars indicate mean ± SEM. Two-way ANOVA followed by Bonferroni post hoc test. **p < 0.01, ***p < 0.001 for the treatment effect.

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