Transcript levels of a 18S rRNA, b GAPDH, c BDNF, and d Arc in samples that were reverse transcribed on Day 1 (n = 6 each) and Day 8 (n = 7 each) after the ethanol and NeuroTrace (EtOH/NT) treatment, or unfixed and unstained (Untreated). The collected DG samples were 150,000 μm2 × 20 μm in volume per sample. Values are expressed as copy number of transcripts. Bars indicate mean ± SEM. Two-way ANOVA followed by Bonferroni post hoc test. **p < 0.01, ***p < 0.001 for the treatment effect.