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Figure 3 | BMC Research Notes

Figure 3

From: Application of NeuroTrace staining in the fresh frozen brain samples to laser microdissection combined with quantitative RT-PCR analysis

Figure 3

ac Correlation of the expression levels between the housekeeping genes in the hippocampal DG region of unfixed and unstained (black) and ethanol-fixed and NeuroTrace-stained (green) tissue sections: a GAPDH vs. 18S rRNA, b β-actin vs. GAPDH, and c 18S rRNA vs. β-actin. Correlation between the expression levels of Map2 and d 18S rRNA, e GAPDH, and f β-actin. Correlation between the expression levels of BDNF and g 18S rRNA, h GAPDH, and i β-actin. Correlation between the expression levels of Arc and j 18S rRNA, k GAPDH, and l β-actin. Correlation between the expression levels of cFos and m 18S rRNA, n GAPDH, and o β-actin. Values are expressed as copy number of transcripts. Both untreated (n = 13) and treated (n = 13) samples were reverse transcribed on Day 1 and Day 8 after the treatment.

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