a H & E-stained pituitary cryosection on a silane-coated microslide. b Ethanol-fixed and NeuroTrace-stained pituitary sections under (b
) bright field and (b
) fluorescence radiated field. c–e Comparative analysis between the anterior and posterior pituitary showing specific transcript expression profile for each region. c corticotropin-releasing hormone receptor 1; CRHR1, d vasopressin; AVP, and e oxytocin; OXT. The transcript levels in the posterior pituitary values (n = 8) were expressed as fold change compared to the anterior pituitary values (n = 9) and after normalization to GAPDH. Bars indicate mean ± SEM. Asterisks (***p < 0.001) indicate statistically significant difference between samples collected from the anterior and posterior pituitary glands, as assessed by Student’s t-test. Scale bars
a, b 310 μm, and c 100 μm.